Combining Channels via Arithmetic Operations

Chromeleon also allows you to combine two channels of different samples, using arithmetic operations. Each data point is created by combining the associated two data points from the existing channels (i.e., the data points at the corresponding time), using the selected operation.

Arithmetic combinations are not restricted to chromatograms in the strict sense. They can be used for all 2D channels, except for temporary channels.

 Tip: 

You may also define the combination of two channels in the Post-Acquisition Steps view of the PGM Editor (see Control  The PGM Editor). Please observe the corresponding hints below.

How To

  1. Display the sample's chromatogram by double-clicking its name in the Browser.

 Tip: 

Open the PGM File in which you want to define that two channels are combined and select the Post-acquisition steps view, instead.

  1. Select Arithmetic combination of channels on the context menu. The Arithmetic combination of channels dialog box appears.

 Tip: 

In the PGM Editor, follow the steps below: Select Insert line on the context menu to add a new post-acquisition step. The New post-acquisition step dialog box is opened. Select Arithmetic combination of channels to open the Arithmetic combination of channels dialog box.

  1. The current channel is Channel A. Select the factor with which the channel shall be multiplied.

 Tip:

In the PGM Editor, select the channel of the corresponding sample from the channels defined in the PGM File. Channels defined in previous post-acquisition steps are also available for selection.

  1. Select a channel of the same sample or of any other sample of your choice as Channel B. Also, determine the factor with which Channel B is multiplied.

  2. Select an Operation.

  3. In the Result Channel box, enter the name for the new channel. The suggested name is the abbreviation for the operation plus the names of the two channels. However, you are free to enter any name of your choice as well.

  4. To combine the selected channels for all samples in the sequence or Query, select the Apply to all samples in the current sequence or query check box.

 Tip: 

This option is not provided in the Arithmetic combination of channels dialog box of the PGM Editor.

  1. Click OK to start the procedure.